Name of the Test:   Prostaglandin E2

Alias Names: PGE2

Clinical Applications:

Prostaglandins are synthesized  from arachidonic acid by cyclooxygenase ( COX)-1 or -2, which converts the acid into PGH2. This is further processed by cytosolic or microsomal prostaglandin synthesis to become PGE2. They are closely related to the Thromboxanes and Leukotrienes. Prostaglandin E2 is metabolized to Prostaglandin  F2 alpha, A2, and Dihydroketo Prostaglandin E2.  Prostaglandin E2 is excreted directly into the urine.  Prostaglandin E2 is a potent vasodilator and also a stimulus for Renin release.  Prostaglandin E2 release is stimulated by cholinergic and alpha adrenergic agents.  Prostaglandin E2 potentiates the actions of Histamine and Bradykinin causing pain and accumulation of edema fluid.  It relaxes the circular muscle of the gut in opposition to Prostaglandin F2 alpha and also relaxes the lower esophageal sphincter.  Prostaglandin E2 also causes accumulation of water and electrolytes in the lumen of the gut by stimulating their secretion.  Elevated levels of Prostaglandin E2 have been detected in patients with the Watery Diarrhea Syndrome, arthritis, fever, tissue injury, endometriosis, neural crest tumors, pheochromocytomas, and other amine-peptide-secreting tumors. Prostaglandin E2 production and circulating levels are drastically suppressed by aspirin and indomethacin.

 Method:

Samples are assayed by an ELISA.

Test Information:

Specimen requirements: 3.0 ml of EDTA plasma or serum (1.0 ml minimum).

Patient Preparation: Fasting specimens are preferred. Patients should be free from medications( like aspirin, and anti-inflammatory drugs) for 2 days.

Shipping requirements: Ship the samples frozen in Dry ice.

Turnaround time:1 week (set up every Tuesday and reported the following day). Repeat samples are assayed the same week .

Reference Range:

200 – 400 pg /ml

 

References:

1.Vane JR(1971) Inhibition of prostaglandin synthesis as a mechanism of action of aspirin like drugs. Nature 231: 231 -235.

2.Hayaishi O ( 1988) Sleep wake regulation by prostaglandins D2 and E2. J Biol Chem 263:14593 -14596.

3.Fiorito S,Magrini L, Goalard C ( 2003) Pro-inflammatory and anti-inflammatory circulating cytokines and periprosthetic osteolysis. J of bone and Joint surgery 85: 1202 -1206.

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